Abstract
Background Context: Mesenchymal stem cells (MSC) are being used for spine and orthopaedic surgical and research applications. Bone marrow and fat are the most commonly used sources of these cells.
Purpose: To describe a new technique allowing the isolation and expansion of human MSC from cortical bone.
Study Design: MSC from human cortical bone (vertebral lamina) were isolated, expanded, and verified in vitro.
Methods: Human MSC were isolated from laminar bone obtained during surgery (decompression/laminectomy). They were then cultured and assessed using fluorescence-activated cell sorting techniques forMSCmarkers, colonyforming unit assays, and multilineage differentiation.
Results: Isolated and cultured cells demonstrated MSC markers and trilineage differentiation confirming their stemness.
Conclusion: Anovel method for the isolation of MSC from cortical bone has been described. These cells have significant current and future application in spine and orthopaedic surgery; and both the source of the cells and particular characteristics of the cortical bone derived MSC have advantages over currently used MSC obtained from other sources.
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