Dexamethasone Induced Modulation of Reproduction: Investigating Role of Dexras1 Monomeric G Protein

Authors

  • Rashmi Verma Department of Biotechnology
  • Navin Kumar Department of Biotechnology
  • Ashish Thapliya Department of Life Sciences, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India

Keywords:

Reproduction, Dexamethasone, Dexras1, Reproduction, Stress, LH, FSH, Glucocorticoids.

Abstract

Many factors like hormonal, anatomical, genetic, and environmental etc effect reproduction. Stressful conditions
like emotional-stress, altered level of hormones etc can cause alterations in release of reproductive hormones
thus ultimately effecting reproduction. In our study, we have focused to know the modulation of reproduction
induced by Dexamethasone (DEX) (a synthetic glucocorticoid), which mimics as a stress condition in female
mice and investigated the role of Dexras1 in reproduction under the influence of Dexamethasone.
Glucocorticoid are known to influence the release of GnRH hormone which in turn regulate the release of
reproductive hormones (LH, FSH). Dexamethasone is reported to upregulates Dexras1 expression. So we have
investigated the modulation of reproduction by alteration in the release of reproductive hormones LH and FSH
under DEX dosage in female mice. We observed that the level of hormones LH and FSH altered, in the
presence of Dexamethasone doses (0.1 and 0.5mg/kg mice). LH level is decreased while the level of FSH
increased with DEX dose. So the present study reveals the role of Dexamethasone in modulation of
reproduction. It has been reported earlier that Dexras1 is upregulated by Dexamethasone so we could suggest
that Dexras1 might involve in modulation of reproduction and could become a very important therapeutic target
in reproduction signaling processes.

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References

Breen, K. M., & Mellon, P. L. (2014). Influence of stress-induced intermediates on gonadotropin gene

expression in gonadotrope cells. Molecular and Cellular Endocrinology, 385, 71–77.

Breen, K. M., Thackray, V. G., Hsu, T., Mak-McCully, R. A., Coss, D., & Mellon, P. L. (2012). Stress levels of

glucocorticoids inhibit LHβ-subunit gene expression in gonadotrope cells. Molecular Endocrinology, 26(10),

-1731.

Dardes, R. C., Baracat, E. C., & Simões, M. J. (2000). Modulation of estrous cycle and LH, FSH and melatonin

levels by pinealectomy and sham-pinealectomy in female rats. Progress in Neuro-Psychopharmacology &

Biological Psychiatry, 24(3), 441-453.

Dolatabadi, A. A., & Zarchii, S. R. (2015). The effect of prescription of different dexamethasone doses on

reproductive system. Biomedical Research, 26(4), 656-660.

Fernandez, I., Pena, A., Del Teso, N., Perez, V., & Rodriguez-Cuesta, J. (2010). Clinical biochemistry

parameters in C57BL/6J mice afterblood collection from the submandibular vein and retroorbital plexus. Journal

of the American Association for Laboratory Animal Science, 49(2), 202–206.

05 0.09

31

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2

3

4

Group 1 Control Group 2 (0.1mg/kg) Group 3 (0.5mg/kg)

FSH ng/ml

Average value

Journal of Graphic Era University

Vol. 8, Issue 1, 27-32, 2020

ISSN: 0975-1416 (Print), 2456-4281 (Online)

Gao, H., Gao, Y., Li, X., Shen, A., & Yan, M. (2010). Spatiotemporal patterns of dexamethasone-induced Ras

protein 1 expression in the central nervous system of rats with experimental autoimmune encephalomyelitis.

Journal of Molecular Neuroscience, 41(1), 198-209.

Gooyande, J., Modaresi, M., & Pirestani, A. (2014). Comparing betamethasone and dexamethasone effects on

concentration of male reproductive hormones in mice. Research Journal of Applied Sciences, Engineering and

Technology, 7(2), 413-416.

Gore, A. C., Attardi, B., & DeFranco, D. B. (2006). Glucocorticoid repression of the reproductive axis: effects

on GnRH and gonadotropin subunit mRNA levels. Molecular and Cellular Endocrinology, 256(1-2), 40-48.

Hong, K., & Choi, Y. (2018). Role of estrogen and RAS signaling in repeated implantation failure. BMB

Reports, 51(5), 225-229.

Kemppainen, R. J., & Behrend, E. N. (1998). Dexamethasone rapidly induces a novel ras superfamily member-

related gene in AtT-20 cells. Journal of Biological Chemistry, 273(6), 3129– 3131.

Kim, H. R., Cho, K. S., Kim, E., Lee, O. H., Yoon, H., Lee, S., Moon, S., Park, M., Hong, K., Na, Y., Shin, J.E.,

Kwon, H., Song, H., Choi, D.H., & Choi, Y. (2017). Rapid expression of RASD1 is regulated by estrogen

receptor-dependent intracellular signaling pathway in the mouse uterus. Molecular and Cellular Endocrinology,

, 32-39.

Li, X., Cheng, C., Fei, M., Gao, S., Niu, S., Chen, M., Liu, Y., Guo, Z., Wang, H., Zhao, J., Yu, X., & Shen, A.

(2008). Spatiotemporal expression of Dexras1 after spinal cord transection in rats. Cellular & Molecular

Neurobiology, 28(3), 371–388.

Schafer, S. C., Wallerath, T., Closs, E. I., Schmidt, C., Schwarz, P. M., Forstermann, U., & Lehr, H. A. (2005).

Dexamethasone suppresses eNOS and CAT-1 and induces moxidative stress in mouse resistance arterioles.

American Journal of Physiology-Heart and Circulatory Physiology, 288, H436–H444.

Shirasaki, Y., Ito, Y., Kikuchi, M., Imamura, Y., & Hayashi, T. (2012). Validation studies on blood collection

from the jugular vein of conscious mice. Journal of the American Association for Laboratory Animal Science,

(3), 345–351.

Soga, T., Dalpatadu, S. L., Wong, D. W., & Parhar, I. S. (2012). Neonatal dexamethasone exposure down-

regulates GnRH expression through the GnIH pathway in female mice. Neuroscience, 218, 56-64

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Published

2023-02-28

How to Cite

Verma, R., Kumar, N., & Thapliya, A. (2023). Dexamethasone Induced Modulation of Reproduction: Investigating Role of Dexras1 Monomeric G Protein. Journal of Graphic Era University, 8(1), 27–32. Retrieved from https://riverpublishersjournal.com/index.php/JGEU/article/view/52

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