In Vitro Evaluation of Anticancer Drugs as Antimicrobial Agent against the Pathogenic Enteric Bacteria

Authors

  • Pranjali Gupta Department of Biotechnology Graphic Era University, Dehradun, India
  • Pankaj Gautam Department of Biotechnology Graphic Era University, Dehradun, India
  • Nishant Rai Department of Biotechnology Graphic Era University, Dehradun, India

Keywords:

Anticancer Drugs, Drug Repurposing, Efflux Pump Inhibitor, Multidrug Resistance, Salmonella Typhi

Abstract

Drug repurposing and drug combination have been suggested as a major strategy to counter the spread of
antimicrobial resistance in clinical and community settings. Drug repurposing explores the potential of existing
drugs for new indications with respect to human disease. Mainstream anticancer drug paclitaxel and vinblastine
were tested as potential efflux pump inhibitors in in vitro assays. Both the anticancer drugs failed to produce
zone of inhibition on MHA plates inoculated with Salmonella Typhi in disk diffusion testing. MIC obtained for
the two drugs were found as 80μg/ml (Paclitaxel) and 160 μg/ml (vinblastine) respectively. When tested for
synergy with kanamycin and ciprofloxacin, paclitaxel and vinblastine resulted in indifference (FICI value =1 for
both the antibiotics). The combination of anticancer and antibiotic drugs showed bacteriostatic nature in the
absence of 3log10 reduction in viable Salmonella CFUs over 24 hrs time period in time kill assay. Whereas the
marginal activity was observed in EtBr cartwheel assay as compared to control. Thus in vitro studies paclitaxel
and vinblastine cannot be repurposed as antimicrobial against Salmonella Typhi.

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Published

2023-02-28

How to Cite

Gupta, P., Gautam, P., & Rai, N. (2023). In Vitro Evaluation of Anticancer Drugs as Antimicrobial Agent against the Pathogenic Enteric Bacteria. Journal of Graphic Era University, 5(2), 155–163. Retrieved from https://riverpublishersjournal.com/index.php/JGEU/article/view/108

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