Production of Taxol by Endophytic Fungi Isolated from Roots of Himalayan Yew (Taxus wallichiana Zucc.)

  • Priyanka Adhikari G.B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora-263643, Uttarakhand, India
  • Mithilesh Singh G.B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora-263643, Uttarakhand, India
  • Anita Pandey 1)G.B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora-263643, Uttarakhand, India 2)Department of Biotechnology, Graphic Era Deemed to be University, Bell Road, Clement Town, Dehradun-248002, Uttarakhand, India
Keywords: Taxus wallichiana, endophytes, taxol, Aspergillus, Penicillium

Abstract

Taxol® (generic name – Paclitaxel), the most promising chemotherapeutic agent was isolated from bark of different Taxus sp. As Taxus species are threatened with extinction (endangered in Himalaya), thus it is imperative to develop alternate and sustainable method for commercialization and scale up production of paclitaxel. In this respect, physical and chemical parameters are effective and important key points for active compound production particularly by using endophytic microbes. In the present study, five endophytic fungi isolated from the roots of Taxus wallichiana, are tested for paclitaxel production using biochemical and molecular methods. Subsequently, effect of physico-chemical parameters like temperature, pH, incubation time, and medium constituents i.e., salt concentration, carbon and nitrogen sources on paclitaxel production were also analyzed. Among isolates, two of the fungi viz. GBPI_TWR F1 (Penicillium sp.) and GBPI_TWR F5 (Aspergillus sp.) were found to be paclitaxel producing. The genomic DNA samples were sequenced to confirm the presence of two genes viz. 10-deacetylbaccatin III-10-O-acetyl transferase (DBAT) and C-13 phenylpropanoid side chain-CoA acyltransferase (BAPT), implicated in paclitaxel biosynthesis. Both the endophytes showed the amplicons of DBAT and BAPT genes. Results revealed that after optimization of medium components and physical condition, paclitaxel production was increased in both the endophytes, maximum paclitaxel production i.e., 5.45 ± 0.98 mg/L was obtained by GBPI_TWR F5 (Aspergillus sp.) following 10 days of incubation at 15∘C in optimized S7 liquid medium composition.

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Author Biographies

Priyanka Adhikari, G.B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora-263643, Uttarakhand, India

Priyanka Adhikari is Research Associate at National Institute of Pharmaceutical Education and Research (NIPER), Guwahati-Assam, India. Before Joining NIPER, Dr. Adhikari worked as Junior and Senior Project Fellow in National Mission on Himalayan Studies (NMHS) Program of Ministry of Environment, Forest and Climate Change, Govt. of India at G.B. Pant National Institute of Himalayan Environment, Almora – Uttarakhand, India. Dr. Adhikari has M.Sc. in Microbiology and Ph.D. in Biotechnology from Kumaun University, Nainital-Uttarakhand, India. Her research interests include plant-microbe interactions and characterization, and identification of microbe and plant based bioactive compounds and drug formulation. Dr Adhikari has been recognized for National Award (twice) from MoEF & CC Himalayan Researchers Consortium and National Medicinal Plant Board (NMPB) Govt. of India for her work on microbiological and biochemical aspects of Taxus wallichiana. Later, she received the Young Scientist Travel Grant to present her work in 8th Conference “FEMS 2019” at Glasgow Scotland, United-Kingdom. She has published more than 20 peer reviewed papers in National and International Journals.

Mithilesh Singh, G.B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora-263643, Uttarakhand, India

Mithilesh Singh is working as Scientist at Centre for Environmental Assessment and Climate Change, G.B. Pant National Institute of Himalayan Environment (GBP-NIHE), Almora, Uttarakhand. She has done post graduation in Botany from Banaras Hindu University and PhD in Biotechnology from IIT Guwahati, Assam. Her field of specialization includes plant biotechnology and bioprospection. She has published 55 research papers, chapters in edited books/proceedings and scientific popular articles having over 520 citation and 12 h index. Dr. Singh has received DBT BioCARe Women Scientist Award in 2014.

Anita Pandey, 1)G.B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora-263643, Uttarakhand, India 2)Department of Biotechnology, Graphic Era Deemed to be University, Bell Road, Clement Town, Dehradun-248002, Uttarakhand, India

Anita Pandey is presently affiliated with the Department of Biotechnology, Graphic Era (Deemed to be University, Dehradun, Uttarakhand, India. Previously, she was Scientist at G.B. Pant National Institute of Himalayan Environment, Almora, India. Dr. Pandey has extensively worked on Bioprospection of Microbial Diversity of Indian Himalayan region (IHR). Her research areas include- Extremophiles, Rhizosphere, Biodegradation, Fermented Foods, and Antimicrobials.

Her scientific contributions have been recognized at State, National, and International levels. She has been recipient of National Bioscientist Award by Department of Biotechnology, Govt. of India and Vishisht Mahila Vaigyanik Sammaan (Eminent Woman Scientist Recognition) by Uttarakhand Science, Education and Research Center, Department of Science and Technology, Govt. of Uttarakhand, India.

Dr. Pandey has hosted CV Raman International Fellowship for African Researchers twice (Morocco and Egypt). A new archeal species (Nantronococcus pandeyae sp. nov.) has been named in her honour for her extensive contribution to microbial diversity of IHR (Current Microbiology, vol. 79, Article number 51, 2022).

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Published
2022-10-06
Section
Articles